Journal: Bioconjugate Chemistry

Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

doi: 10.1021/acs.bioconjchem.3c00406

Figure Lengend Snippet: Characterization of SCC4-EGFP spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).

Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

Techniques:

Journal: Bioconjugate Chemistry

Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

doi: 10.1021/acs.bioconjchem.3c00406

Figure Lengend Snippet: Fluorescence imaging of DFAC-treated SCC4 spheroids. (A) Experimental scheme of SCC4-EGFP 3D spheroid treatment with DFAC and imaging. (B) IVIS fluorescence imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned at the top, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the left. (C) Quantification of fluorescence signals from the IVIS images is represented as the tumor spheroid-to-background ratio. Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way ANOVA with Tukey’s test for post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

Techniques: Fluorescence, Imaging

Journal: Bioconjugate Chemistry

Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

doi: 10.1021/acs.bioconjchem.3c00406

Figure Lengend Snippet: Photoacoustic imaging of DFAC-treated SCC4 spheroids. (A) Photoacoustic imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned on the left, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the top. (B) Quantification of photoacoustic signals from the images represented as the normalized photoacoustic signal intensity per mm 3 . Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way Brown–Forsythe and Welch ANOVA test with post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

Techniques: Imaging

Journal: Bioconjugate Chemistry

Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

doi: 10.1021/acs.bioconjchem.3c00406

Figure Lengend Snippet: Long-term response of SCC4 spheroids to DFAC-mediated PIT. (top) Experimental schedule for the long-term response of SCC4 spheroids to PIT. Cells were seeded in CellCarrier Spheroid ULA 96-well microplates, followed by incubation with DFAC for 24 h. The spheroids were then irradiated with varying light doses of 10–100 J/cm 2 , followed by imaging for 5 weeks. (bottom left) Maximum intensity projection (MIP) of fluorescence signals from SCC4 spheroids on day 31 post-PDT. Scale bar corresponds to 500 μm. (bottom right) Relative viability of SCC4 spheroids treated with PIT is shown in the right panel.

Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

Techniques: Incubation, Irradiation, Imaging, Fluorescence

Journal: Bioconjugate Chemistry

Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

doi: 10.1021/acs.bioconjchem.3c00406

Figure Lengend Snippet: Recurrence of SCC4 spheroid growth after DFAC-mediated PIT. SCC4 spheroids with different volumes were treated with PIT with different light doses: (A) 5 J/cm 2 , (B) 25 J/cm 2 , (C) 50 J/cm 2 , and (D) 100 J/cm 2 .

Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

Techniques: